Australian centre for genomic epidemiological microbiology

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Publications Archive


Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Trussart M, Yus E, Martinez S, Bau D, Tahara YO, Pengo T, Widjaja M, Kretschmer S, Swoger J, Djordjevic S, Turnbull L, Whitchurch C, Miyata M, Marti-Renom MA, Lluch-Senar M, Serrano L. Nat Commun. 2017 Mar 8;8:14665. doi: 10.1038/ncomms14665.

Genomic islands 1 and 2 carry multiple antibiotic resistance genes in Pseudomonas aeruginosa ST235, ST253, ST111 and ST175 and are globally dispersed

Roy Chowdhury P, Scott M, Djordjevic SP. Journal of Antimicrobial Chemotherapy. 2017 Feb 72 (2): 620-622. doi:


Cross reactivity among the swine mycoplasmas as identified by protein microarray

Petersen AC, Oneal DC, Seibel JR, Poel K, Dauma CL, Djordjevic SP, Minion FC. Veterinary Microbiology. 2016 Aug 30;192:204-12. doi: 10.1016/j.vetmic.

The quest for improved reproducibility in MALDI mass spectrometry

O’Rourke MB, Djordjevic SP, Padula MP. Mass Spectrom Rev. 2016 Jul 15. doi: 10.1002/mas.21515.

Genomic Microbial Epidemiology Is Needed to Comprehend the Global Problem of Antibiotic Resistance and to Improve Pathogen Diagnosis

Wyrsch ER, Roy Chowdhury P, Chapman TA, Charles IG, Hammond JM, Djordjevic SP. Front Microbiol. 2016 Jun 15;7:843. doi: 10.3389/fmicb.

Comparative genomic analysis of toxin-negative strains of Clostridium difficile from humans and animals with symptoms of gastrointestinal disease

Roy Chowdhury P, DeMaere M, Chapman T, Worden P, Charles IG, Darling AE, Djordjevic SP. BMC Microbiol. 2016 Mar 16(1):41. doi: 10.1186/s12866-016-0653-3.

Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa

Roy Chowdhury P, Scott M, Worden P, Huntington P, Hudson B, Karagiannis T, Charles IG, Djordjevic SP. Open Biol. 2016 Mar;6(3). pii: 150175. doi: 10.1098/rsob.150175.

The Role of CD44 and ERM Proteins in Expression and Functionality of P-glycoprotein in Breast Cancer Cells

Pokharel D, Padula MP, Lu JF, Jaiswal R, Djordjevic SP, Bebawy M. Molecules. 2016 Mar 1;21(3):290. doi: 10.3390/molecules21030290.

Post-translational processing targets functionally diverse proteins in Mycoplasma hyopneumoniae

Tacchi JL., Raymond BBA, Haynes PA, Berry IJ, Widjaja M, Bogema DR, Woolley LK, Jenkins C, Minion FC, Padula MP, Djordjevic SP. Open Biology. 2016 Feb;6(2):150210. doi: 10.1098/rsob.150210.

The application of terminomics for the identification of protein start sites and proteoforms in bacteria

Berry IJ, Steele JR, Padula MP, Djordjevic SP. Proteomics. 2016 Jan;16(2):257-72. doi: 10.1002/pmic.201500319.


Non-proteolytic functions of microbial proteases increases pathological complexity

Jarocki VM, Tacchi JL, Djordjevic, SP. Proteomics. 2015. Dec 10. doi: 10.1002/pmic.201400386.

P40 and P90 from Mpn142 are Targets of Multiple Processing Events on the Surface of Mycoplasma pneumonia

Widjaja M., Berry I, Pont E, Padula M, Djordjevic SP. Proteomes. 2015 Dec; 3(4): 512–537. doi: 10.3390/proteomes3040512.

A non-instrument-based method for the analysis of formalin-fixed paraffin-embedded human spinal cord via matrix-assisted laser desorption/ionisation imaging mass spectrometry

O’Rourke MB, Djordjevic SP, Padula MP. Rapid Commun Mass Spectrom. 2015 Oct 15;29(19):1836-40. doi: 10.1002/rcm.7283.

Exploitation of plasmin(ogen) by bacterial pathogens of veterinary significance

Raymond BBA, Djordjevic SP. Veterinary Microbiology. 2015 Jul 9;178(1-2):1-13. doi: 10.1016/j.vetmic.

Tn6026 and Tn6029 are found in complex resistance regions mobilised by diverse plasmids and chromosomal islands in multiple antibiotic resistant Enterobacteriaceae

Reid C, Roy Chowdhury P, Djordjevic SP. Plasmid. 2015 Jul;80:127-37. doi: 10.1016/j.plasmid.

Temporal dynamics and subpopulation analysis of Theileria orientalis genotypes in cattle

Jenkins C, Micallef M, Alex SM, Collins D, Djordjevic SP, Bogema DR. Infection, Genetics & Evolution. 2015 Jun;32:199-207. doi: 10.1016/j.meegid.

Comparative Genomic Analysis of a Multiply Antimicrobial Resistant Enterotoxigenic E. coli O157 Lineage from Australian Pigs

Wyrsch E, Roy Chowdhury P, Abraham S, Santos J, Darling A, Charles IG, Chapman T, Djordjevic SP. BMC Genomics. 2015 Mar 10;16:165. doi: 10.1186/s12864-015-1382-y.

A Versatile Cost Effective Method For The Analysis of Fresh Frozen Tissue Sections Via Matrix Assisted Laser Desorption Ionisation Imaging Mass Spectrometry (MALDI-IMS)

O’Rourke M, Djordjevic SP, Padula MP. Rapid Communications in Mass Spectrometry. 2015 Mar 29(7):637-644. doi: 10.1002/rcm.7138.

Roy Chowdhury P, Charles IG, Djordjevic SP. PLoS One. 2015 Feb 12; 10(2):e0115781. doi: 10.1371/journal.pone.0115781.

MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae

Jarocki VM, Santos J, Tacchi JL, Raymond BBA, Deutscher AT, Jenkins C, Padula MP, Djordjevic SP. Open Biology. 2015 Jan 5(1). pii: 140175. doi: 10.1098/rsob.140175.

Mycoplasmal surface-associated aminopeptidases are multifunctional moonlighting proteins

Jarocki VM, Padula MP, Djordjevic SP. Inflammation and Cell Signaling. 2015 2: e828. doi: 10.14800/ics.828.


Proteolytic processing of the cilium adhesin MHJ_0194 (P123J ) in Mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules 

Raymond BB, Jenkins C, Seymour LM, Tacchi JL, Widjaja M, Jarocki VM, Deutscher AT, Turnbull L, Whitchurch CB, Padula MP, Djordjevic SP. Cellular Microbiology. 2014 Oct 3. doi: 10.1111/cmi.12377

A draft genome of Escherichia coli sequence Type 127 Strain 2009-46

Darling AE, McKinnon J, Worden P, Santos J, Charles IG, Roy Chowdhury P, Djordjevic SP. Gut Pathogens. 2014 Sept 1;6:32. doi: 10.1186/1757-4749-6-32.

Proteome Analysis of Multidrug Resistant Breast Cancer Derived Microparticles

Pokharel D, Padula MP, Lu JF, Tacchi JL, Luk F, Djordjevic SP, Bebawy M. Journal of Extracellular Vesicles. 2014 Aug 22;3. doi: 10.3402/jev.v3.24384.

Genomic interplay in bacterial communities: Implications for growth promoting practices in animal husbandry

Roy Chowdhury P, McKinnon J, Wyrsch E, Hammond JM, Charles IG, Djordjevic SP. Front Microbiol. 2014 Aug 12;5:394. doi: 10.3389/fmicb.2014.00394.

Proteogenomic mapping of Mycoplasma hyopneumoniae virulent strain 232

Pendarvis K, Padula MP, Tacchi JL, Petersen AC, Djordjevic SP, Burgess SC, Minion FC.  BMC Genomics. 2014 Jul 8;15(1):576. doi: 10.1186/1471-2164-15-576.

Evaluation of recombinant Mycoplasma hyopneumoniae P97/P102 paralogs formulated with selected adjuvants as vaccines against Mycoplasmal pneumonia in pigs

Woolley LK, Fell SA Gonsalves JR, Raymond BB, Collins, D, Kuit TA, Walker MJ, Djordjevic SP, Eamens GJ, Jenkins C. Vaccine. 2014 Jun 12;32(34):4333-41. doi: 10.1016/j.vaccine.2014.06.008.

Cilium adhesim P216 (MHJ_0493) is a target of ectodomain shedding and aminopeptidase activity on the surface of Mycoplasma hyopneumoniae

Tacchi JL, Raymond BB, Jarocki VM, Berry IJ, Padula MP, Djordjevic SP. Journal of Proteome Research. 2014 Jun 6;13(6):2920-30. doi: 10.1021/pr500087c

The genome of Clostridium difficile 5.3

Darling AE, Worden P, Chapman TA, Roy Chowdhury P, Charles IG, Djordjevic SP. Gut Pathogens. 2014 Feb 24;6:4. doi:10.1186/1757-4749-6-4.

Comparative proteomics and glycoproteomics reveal increased N-linked glycosylation and relaxed sequon specificity in Campylobacter jejuni NCTC11168 O

Scott NE, Marzook NB, Cain JA, Solis N, Thaysen-Andersen M, Djordjevic SP, Packer NH, Larsen MR, Cordwell SJ. Journal of Proteome Research. 2014 13(11):5136-50. doi: 10.1021/pr5005554.

Chapter 5: Post-translational modification of proteins in the Mollicutes

Djordjevic SP and Tacchi JL. In Mollicutes: Molecular Biology & Pathogenesis. 2014. Editors: Browning GF & Citti C. Caister Academic Press.


P159 from Mycoplasma hyopneumoniae binds porcine cilia and heparin and is cleaved in a manner akin to ectodomain shedding

Raymond BBA, Tacchi JL, Jarocki VM, Minion FC, Padula MP, & Djordjevic SP. Journal of Proteome Research. 2013 Dec 6;12(12):5891-903. doi: 10.1021/pr400903s.

Sequences of two related multiple antibiotic resistance virulence plasmids sharing a unique IS 26-related molecular signature isolated from different Escherichia coli pathotypes from different hosts 

Venturini C, Hassan KA, Roy Chodhury P, Paulsen IT, Walker MJ, Djordjevic SP. PLoS ONE. 2013 Nov 4;8:11. doi: 10.1371/journal.pone.0078862.

Plasmin activity in the porcine airways is enhanced during experimental infection with Mycoplasma hyopneumoniae, is positively correlated with proinflammatory cytokine levels and is ameliorated by vaccination

Woolley LK, Fell S, Djordjevic SP, & Eamens GJ, Jenkins C. Veterinary Microbiology. 2013 May 31;164(1-2):60-6. doi: 10.1016/j.vetmic.2013.02.003.

Mobile elements, zoonotic pathogens and commercial bacteria: conduits for the delivery of resistance genes into humans, production animals and soil microbiota

Djordjevic SP, Stokes HW, Roy Chowdhury P. Front Microbiol. 2013 Apr 30;4.86. doi:10.3389/fmicb.2013.00086. Print 2013

MHJ_0125 is a M42 glutamyl aminopeptidase that moonlights as a multifunctional adhesin on the surface of Mycoplasma hyopneumoniae

Robinson MW, Buchtmann KA, Jenkins C, Tacchi JL, Raymond BBA, To J, Roy Chowdhury P, Woolley LK, Labbate M, Turnbull L, Whitchurch CB, Padula MP, Djordjevic SP. Open Biology. 2013 Apr 17;3(4):130017. doi: 10.1098/rsob.130017.

Deletion of Integron-associated gene cassettes impact on the surface properties of Vibrio rotiferianus DAT722

Rapa R, Shimmon R, Djordjevic SP, Stokes HW, Labbate M. PLoS One. 2013 Mar 6;8(3):e58430. doi:10.1371/journal.pone.0058430.

Indigenous Vibrio cholerae strains from a non-endemic region are pathogenic

Islam A, Labbate M, Alam M, Djordjevic SP, Darling A, Melvold J, Holmes AJ, Johura FT, Cravioto A, Charles IG, Stokes HW. Open Biology.  2013 Feb 13;3(2):120181. doi: 10.1098/rsob.120181.

Chapter 4: mobilized integrons: team players in the spread of antibiotic resistance genes

Martinez E, Djordjevic SP, Stokes HW, and Roy Chowdhury P. In Lateral Gene Transfer in Evolution. 2013. Pages 79-103. Edited by Uri Gophna. Springer Books. doi: 10.1007/978-1-4614-7780-8_4.


Evaluation of clinical, histological and immunological changes and qPCR detection of Mycoplasma hyopneumoniae in tissues during the early stages of mycoplasmal pneumonia in pigs after experimental challenge with two field isolates

Woolley LK, Fell S, Gonsalves JR, Walker MJ, Djordjevic SP, Jenkins C & Eamens GJ. Veterinary Microbiology. 2012 Dec 28;161(1-2)186-195. Doi:10.1016/j.vetmic.2012.07.025.

Identification of lipoprotein MslA as a neoteric virulence factor of Mycoplasma gallisepticum

Szczepanek SM, Frasca S Jr, Schumacher VL, Liao X, Padula M, Djordjevic SP, Geary SJ. Infection & Immunity. 2012 June 1;78: 3475-3483. doi: 10.1128/IAI.00154-10.

Molecular characterization of 21.4 kilobase antibiotic resistance plasmid from an ?-hemolytic Escherichia coli O108:H- human clinical isolate

Dawes F, Bulach DM, Kuzevski A, Bettelheim KA, Venturini C, Djordjevic SP, Walker MJ. PLoS One. 2012 Apr 20;7(4):e34718. doi: 10.1371/journal.pone.0034718.

Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a novel mechanism by which Mycoplasma hyopneumoniae regulates surface topography

Bogema DR, Deutscher AT, Woolley LK, Seymour LM, Raymond BB, Tacchi JL, Padula MP, Dixon NE, Minion CF, Jenkins C, Walker MJ, Djordjevic SP.  mBio. 2012 Apr 3;3(2)e00282-11. doi: 10.1128/mBio.00282-11.

Class 1 integron associated spread of resistance regions in Pseudomonas aeruginosa: plasmid or chromosomal platforms?

Stokes HW, Martinez E, Roy Choudhuri P, Djordjevic SP.  J. Antimicrob. Chemother.  2012 Mar 28;67(7):1799-1800. doi: 10.1093/jac/dks116.

Mycoplasma hyopneumoniae surface proteins Mhp385 and Mhp384 bind host cilia and glycosaminoglycans and are endoproteolytically processed by proteases that recognize different cleavage motifs

Deutscher AT, Tacchi JL, Minion FC, Padula MP, Crossett B, Bogema DR, Jenkins C, Kuit TA, Walker MJ, Djordjevic SP. Journal of Proteome Research. 2012 Mar 2;11(3): 1924-1936. doi: 10.1021/pr201115v.

Diverse mobilized Class 1 integrons are common in the chromosome of pathogenic Pseudomonas aeruginosa clinical strains

Martinez E, Marquez C, Ingold A, Merlino J, Djordjevic SP, Stokes HW, Roy Chowdhury P. Antimicrob. Agents Chemother. 2012 Jan 23;56(4):2169-2172. doi: 10.1128/AAC.06048-11.

Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface 

Seymour LM, Jenkins C, Deutscher AT, Raymond BB, Padula MP, Tacchi JL, Bogema DR, Eamens GJ, Woolley LK, Dixon NE, Walker MJ, Djordjevic SP. Cellular Microbiology. 2012 Jan 14;14(1):81-94. doi: 10.1111/j.1462-5822.2011.01702.x

Mass spectrometric characterization of the Campylobacter jejuni adherence factor CadF reveals post-translational processing that removes immunogenicity while retaining fibronectin binding

Scott NE, Marzook NB, Deutscher A, Falconer L, Crossett B, Djordjevic SP, Cordwell SJ. Proteomics. 2012 Jan 10;10:277-288. doi: 10.1002/pmic.200900440.


The sequence TTKF | QE defines the site of proteolytic cleavage in Mhp683, a novel glycosaminoglycan and cilium adhesin of Mycoplasma hyopneumoniae

Bogema DR, Scott NE, Padula M, Tacchi JL, Raymond BB, Jenkins C, Cordwell SJ, Minion FC, Walker MJ, Djordjevic SP. Journal of Biological Chemistry. 2011 Dec 2;286(14):41217-41229. doi: 10.1074/jbc.M111.226084.

Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae

Seymour LM, Falconer L, Deutscher AT, Minion FC, Padula MP, Dixon NE, Djordjevic SP, Walker MJ. Journal of Biological Chemistry. 2011 Mar 25;286(12):10097-104. doi: 10.1074/jbc.M110.208140.

Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, HCD and ETD-MS applied to the N-linked glycoproteome of Campylobacter jejuni

Scott NE, Parker BL, Connolly AM, Paulech J, Edwards AVG, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen ML, Cordwell SJ. Molecular & Cellular Proteomics. 2011 Feb 10;(2): M000031MCP201. doi: 10.1074/mcp.M000031-MCP201.


RSF1010-like plasmids in Australian Salmonella enterica serovar Typhimurium and origin of their sul2strAstrB antibiotic gene cluster

Yau S., Liu X, Djordjevic SP, Hall RM. Microbial Drug Resistance. 2010 Dec;16(4):249-52. doi: 10.1089/mdr.2010.0033.

A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia

Seymour LM, Deutscher AT, Jenkins C, Kuit TA, Falconer L, Minion FC, Crossett B, Padula M, Dixon NE, Djordjevic SP, & Walker MJ. Journal of Biological Chemistry.  2010 Oct 29;285(44):33971-33978. doi: 10.1074/jbc.M110.104463.

Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia

Deutscher AT, Jenkins C, Minion FC, Seymour LM, Padula MP, Dixon NE, Walker MJ, Djordjevic SP. Molecular Microbiology. 2010 Oct;78:444-458. doi: 10.1111/j.1365-2958.2010.07345.x.

Transposons related to Tn1696 in IncHI2 plasmids in multiply antibiotic resistant Salmonella enterica serovar Typhimurium from Australian animals 

Cain AK, Liu X, Djordjevic SP, Hall RM. Microbial Drug Resistance. 2010 Sept 16;16:197-202. doi: 10.1089/mdr.2010.0042.

Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid

Venturini, C., S.A. Beatson, S. P. Djordjevic, M.J. Walker. The FASEB Journal. 2010 Apr 24;24:1160-1166. doi: 10.1096/fj.09-144972.


Class 1 integrons with IS26-mediated deletions in their 3´ -conserved segments are widespread in clinical Escherichia coli of human and animal origin

Dawes FE, Kuzevski A, Bettelheim KA, Hornitzky MA, Djordjevic SP, & Walker MJ. PLoS One. 2009 June 15;5(9):e12754.doi: 10.1128/AAC.00294-09.